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Gitmind: https://gitmind.com/app/docs/m5ofx4jo

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🚨 Check List

Metadata

• Including
• Excluding

Amplify region

• V3-V4
• V4
• V3-V5
• V3
• V1-V2
• V1-V3
• V1-V4
• V6

Data preprocess

• Default (recommended)

  Minimum sequence length: 100 bps Maximum sequence length: not specified Maximum ambiguous base: 8 bps Maximum Homopolymer: 8 bps Sequence read: >10,000 read Remove duplicate sequence Remove chimera: use chimera.vsearch Remove contaminants: mitochondria, chloroplasts, unknown, eukaryota and archaea

• Customs Please contacts

Taxonomic profiling

• OTU (OTUs are often used for diversity abalysis)